Removal of contaminating DNA from commercial nucleic acid extraction kit reagents.
نویسندگان
چکیده
Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained.
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عنوان ژورنال:
- Journal of microbiological methods
دوره 61 2 شماره
صفحات -
تاریخ انتشار 2005